EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
   1.1.1.45 | -999 |
- |
more |
kinetic studies |
286905, 286907 |
| 1.1.1.45 | -999 |
- |
more |
no activity with 5-40 mM sugars or hydroxyacids like 2-keto-L-guluonate, 3-deoxyglucosone, xylitol, L-threitol, ascorbic acid, malic acid, L-threonine, L-glycerate, L-tartarate and L-lactate, or 50 mM ethyl and methyl esters of 3-hydroxybutyrate |
669147 |
| 1.1.1.45 | 0.0005 |
- |
NADH |
at pH 8.5, rabbit liver GDH |
669147 |
| 1.1.1.45 | 0.0006 |
- |
NADH |
at pH 8.5, recombinant rabbit GDH |
669147 |
| 1.1.1.45 | 0.01 |
- |
NAD+ |
at pH 8.5, recombinant rabbit GDH |
669147 |
| 1.1.1.45 | 0.01 |
- |
NAD+ |
wild-type enzyme, pH 7.0, 25°C |
712765 |
| 1.1.1.45 | 0.011 |
- |
NAD+ |
at pH 8.5, rabbit liver GDH |
669147 |
| 1.1.1.45 | 0.018 |
- |
L-gulonate |
with NAD+, wild-type enzyme, pH 7.0, 25°C |
712765 |
| 1.1.1.45 | 0.028 |
- |
NAD+ |
mutant enzyme Q41N, pH 7.0, 25°C |
712765 |
| 1.1.1.45 | 0.04 |
- |
NAD+ |
cosubstrate L-gulonate, pH 8.5 |
286905 |
