EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
3.6.1.25 | 0.8 |
- |
ATP |
His-tagged wild type enzyme, at pH 8.1 and 50°C, in the presence of 10 mM Mn2+ |
719965 |
3.6.1.25 | 1.2 |
- |
ATP |
His-tagged mutant enzyme K8A, at pH 10.1 and 50°C, in the presence of 10 mM Mn2+ |
719965 |
3.6.1.25 | -999 |
- |
more |
no change in activity after nitrosylation of the enzyme with 250 microM nitrosylated glutathione |
669193 |
3.6.1.25 | 0.02 |
- |
tripolyphosphate |
wildtype, 2 mM substrate |
669193 |
3.6.1.25 | 0.021 |
- |
tripolyphosphate |
His-tagged wild type enzyme, at pH 9.7 and 37°C, in the presence of 5 mM Mg2+ |
719965 |
3.6.1.25 | 0.04 |
- |
tripolyphosphate |
His-tagged wild type enzyme, at pH 9.7 and 50°C, in the presence of 5 mM Mg2+ |
719965 |
3.6.1.25 | 0.058 |
- |
tripolyphosphate |
His-tagged wild type enzyme, at pH 7.1 and 37°C, in the presence of 5 mM Mg2+ |
719965 |
3.6.1.25 | 0.078 |
- |
tripolyphosphate |
R265H mutant, 2 mM substrate in the presence of 5 mM ATP |
669193 |
3.6.1.25 | 0.083 |
- |
tripolyphosphate |
R265H mutant, 2 mM substrate |
669193 |
3.6.1.25 | 0.1 |
- |
tripolyphosphate |
untagged wild type enzyme, at pH 9.7 and 50°C, in the presence of 5 mM Mg2+ |
719965 |