EC Number   |
KM Value [mM] |
KM Value Maximum [mM]  |
Substrate |
Reference |
|---|
  3.2.1.B41 | -999 |
- |
4-nitrophenyl beta-D-glucopyranoside |
Km-values decrease with an increase in temperature, whereas the kcat values increase, so the catalytic efficiency (kcat/Km) increases 70fold between 40°C and 80°C |
748250 |
| 3.2.1.B41 | -999 |
- |
more |
when the kinetics are performed below 60°C, the enzyme is inhibited by high substrate concentrations. At temperatures higher than 60°C, the inhibition phenomenon is no longer observed but, on the contrary, an activation is obtained at high substrate concentrations. Around 60°C, the enzyme displays Michaelian behavior |
747918 |
| 3.2.1.B41 | 0.08 |
- |
4-nitrophenyl beta-D-fucopyranoside |
80°C, pH 8.5 |
748250 |
| 3.2.1.B41 | 0.1 |
- |
4-nitrophenyl beta-D-glucoside |
60°C, pH 7.0 |
747918 |
| 3.2.1.B41 | 0.12 |
- |
4-nitrophenyl beta-D-fucoside |
60°C, pH 7.0 |
747918 |
| 3.2.1.B41 | 0.16 |
- |
4-nitrophenyl beta-D-glucopyranoside |
80°C, pH 8.5 |
748250 |
| 3.2.1.B41 | 0.28 |
- |
4-nitrophenyl beta-D-glucopyranoside |
70°C, pH 8.5 |
748250 |
| 3.2.1.B41 | 0.39 |
- |
4-nitrophenyl beta-D-glucopyranoside |
60°C, pH 8.5 |
748250 |
| 3.2.1.B41 | 0.83 |
- |
4-nitrophenyl beta-D-glucopyranoside |
50°C, pH 8.5 |
748250 |
| 3.2.1.B41 | 1.74 |
- |
4-nitrophenyl beta-D-galactopyranoside |
80°C, pH 8.5 |
748250 |
