EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
2.6.1.85 | 0.0016 |
- |
chorismate |
apparent K0.5 of chorismate for the activation of glutamine amidotransferase (calculated from chorismate-dependent activation), pH 8.0, 37°C |
724111 |
2.6.1.85 | 0.0022 |
- |
chorismate |
p-aminobenzoate or pyruvate production in the presence of glutamine (assay contains 4-amino-4-desoxychorismate lyase), pH 8.0, 37°C |
724111 |
2.6.1.85 | 0.009 |
- |
chorismate |
NH3-dependent p-aminobenzoate production in the absence of glutamine (assay contains 4-amino-4-desoxychorismate lyase), pH 8.0, 37°C |
724111 |
2.6.1.85 | 0.013 |
- |
chorismate |
wild-type, cosubstrate L-glutamine, pH 8.5, 25°C |
661933 |
2.6.1.85 | 0.02 |
- |
chorismate |
cosubstrate L-glutamine, pH 7.0, 25°C |
724464 |
2.6.1.85 | 0.045 |
- |
L-glutamine |
glutamate production in the presence of chorismate, pH 8.0, 37°C |
724111 |
2.6.1.85 | 0.05 |
- |
L-glutamine |
glutamate production in the absence of chorismate, pH 8.0, 37°C |
724111 |
2.6.1.85 | 0.058 |
- |
chorismate |
wild-type, cosubstrate NH4+, pH 8.5, 25°C |
661933 |
2.6.1.85 | 0.064 |
- |
L-glutamine |
p-aminobenzoate or pyruvate production in the presence of glutamine (assay contains 4-amino-4-desoxychorismate lyase), pH 8.0, 37°C |
724111 |
2.6.1.85 | 0.097 |
- |
chorismate |
mutant K274A, cosubstrate NH4+, pH 8.5, 25°C |
661933 |