EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
   2.3.2.23 | -999 |
- |
more |
analysis of binding affinity between O6-methylguanine-DNA methyltransferase (MGMT)and E2 ubiquitin conjugating enzymes |
758765 |
| 2.3.2.23 | -999 |
- |
more |
initial rates for UBL transfer from the E1 to the E2 enzymes are determined at different E2 concentrations and are fitted using non-linear regression to the Michaelis-Menten model or to a substrate inhibition effect equation. Data, fitted to the Michaelis-Menten model, shows that wild-type UBE2Z, and mutants UBE2ZLB and UBE2ZNter display similar catalytic rates during ubiquitin loading, Enzyme kinetics for UBL loading on UBE2Z variants, overview |
759478 |
| 2.3.2.23 | -999 |
- |
more |
Michaelis constants for wild-type and mutant E2-E3 pairs, E2 is Ube2R2 and E3 is Rbx1, overview |
759768 |
| 2.3.2.23 | 0.000073 |
- |
[ubiquitin-carrier-protein Ubc2b]-L-cysteine |
mutant D576N, pH 7.5, temperature not specified in the publication |
674561 |
| 2.3.2.23 | 0.000089 |
- |
[ubiquitin-carrier-protein Ubc2b]-L-cysteine |
mutant K528A, pH 7.5, temperature not specified in the publication |
674561 |
| 2.3.2.23 | 0.000111 |
- |
[ubiquitin-carrier-protein Ubc2b]-L-cysteine |
wild-type, pH 7.5, temperature not specified in the publication |
674561 |
| 2.3.2.23 | 0.000122 |
- |
[ubiquitin-carrier-protein Ubc2b]-L-cysteine |
mutant D576E, pH 7.5, temperature not specified in the publication |
674561 |
| 2.3.2.23 | 0.000135 |
- |
[ubiquitin-carrier-protein Ubc2b]-L-cysteine |
mutant D576A, pH 7.5, temperature not specified in the publication |
674561 |
| 2.3.2.23 | 0.0019 |
- |
[ubiquitin carrier protein Ubc4]-L-cysteine |
pH 7.5, 25°C |
728208 |
| 2.3.2.23 | 0.2203 |
- |
[UBE2W]-S-ubiquitinyl-L-cysteine |
37°C, pH not specified in the publication |
776614 |
