EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
   3.4.21.62 | 0.0036 |
- |
Suc-Ala-Ala-Pro-Phe-4-nitroanilide |
2.2 mol lactose bound per 1 mol subtilisin, in 10 mM potassium phosphate buffer, pH 7.8, at 25°C |
707805 |
| 3.4.21.62 | 0.0037 |
- |
Suc-Ala-Ala-Pro-Phe-4-nitroanilide |
3.5 mol lactose bound per 1 mol subtilisin, in 10 mM potassium phosphate buffer, pH 7.8, at 25°C |
707805 |
| 3.4.21.62 | 0.0037 |
- |
Suc-Ala-Ala-Pro-Phe-4-nitroanilide |
subtilisin without bound lactose, in 10 mM potassium phosphate buffer, pH 7.8, at 25°C |
707805 |
| 3.4.21.62 | 0.0054 |
- |
benzyloxycarbonyl-L-Ala-L-Ala-L-Leu-4-nitroanilide |
- |
667756 |
| 3.4.21.62 | 0.039 |
- |
L-Ala-L-Ala-L-Val-L-Ala |
wild-type, pH 9.0, 24°C |
670743 |
| 3.4.21.62 | 0.053 |
- |
L-Ala-L-Ala-L-Val-L-Ala |
V104S/L124M/P129S/S130G/P131E/A133S/T134L/L135I, pH 9.0, 24°C |
670743 |
| 3.4.21.62 | 0.059 |
- |
casein |
pH 10, 45°C, Vmax: 0.336 mg/min |
717205 |
| 3.4.21.62 | 0.07 |
- |
succinyl-Ala-Ala-Pro-Phe-4-nitroanilide |
pH 7.5, 5°C |
649692 |
| 3.4.21.62 | 0.09 |
- |
Urea-denatured hemoglobin |
30°C, pH 8.0 |
668316 |
| 3.4.21.62 | 0.11 |
- |
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
subtilisin-like serine protease, at 20°C, in 50 mM Tris-HCl buffer, pH 7.5 |
710470 |
