EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
1.1.1.270 | 0.0107 |
- |
dehydroepiandrosterone |
pH 7.4, 27°C, chimeric 195P-1 mutant |
763397 |
1.1.1.270 | 0.011 |
- |
Pyridine-3-aldehyde |
pH 7.4, 25°C, wild-type enzyme and mutant F303Q |
724124 |
1.1.1.270 | 0.0124 |
- |
dehydroepiandrosterone |
pH 7.4, 27°C, chimeric S194G-1 mutant |
763397 |
1.1.1.270 | 0.013 |
- |
5beta-pregnane-21-ol-3,20-dione |
pH 7.4, 25°C, mutant F303Q |
724124 |
1.1.1.270 | 0.017 |
- |
5beta-dihydrotestosterone |
pH 7.4, 25°C, mutant F303Q |
724124 |
1.1.1.270 | 0.02 |
- |
5beta-dihydrotestosterone |
pH 7.4, 25°C, mutant M304S |
724124 |
1.1.1.270 | 0.0205 |
- |
NAD+ |
pH 7.4, 27°C, chimeric 195P-1 mutant |
763397 |
1.1.1.270 | 0.022 |
- |
Pyridine-3-aldehyde |
pH 7.4, 25°C, mutant L116F |
724124 |
1.1.1.270 | 0.023 |
- |
5beta-pregnane-21-ol-3,20-dione |
pH 7.4, 25°C, mutant M304S |
724124 |
1.1.1.270 | 0.0244 |
- |
NAD+ |
pH 7.4, 27°C, chimeric S194G-1 mutant |
763397 |