EC Number   |
Inhibitors |
Structure |
|---|
    3.2.1.22 | (2S,3R,4R,5S)-2-(hydroxymethyl)-5-[(methylamino)methyl]pyrrolidine-3,4-diol |
- |
   |
| 3.2.1.22 | (2S,3R,4R,5S)-2-[(ethylamino)methyl]-5-(hydroxymethyl)pyrrolidine-3,4-diol |
- |
|
| 3.2.1.22 | 1-deoxy-L-altronojirimycin hydrochloride |
1000fold less effective than 1-deoxygalactonojirimycin |
|
| 3.2.1.22 | 1-deoxygalactonojirimycin |
IC50: 63 nM with mutant enzyme S65T, 48 nM with mutant enzyme S65A, 58 nM with mutant enzyme S65D and 59 nM with wild-type enzyme |
|
| 3.2.1.22 | 1-deoxygalactonojirimycin |
- |
|
| 3.2.1.22 | 1-deoxygalactonojirimycin |
binding to the enzyme protein is enthalpy-driven and stronger than binding of galactostatin bisulfite. Inhibitor fits to the active-site pocket and undergoes hydrogen bonding with residues comprising the active-site pocket including the catalytic ones |
|
| 3.2.1.22 | 1-deoxygalactonojirimycin |
1% residual activity at 0.001 mM |
|
| 3.2.1.22 | 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide |
31% residual activity at 100 mM, at pH 6.0 |
|
| 3.2.1.22 | 1-ethyl-3-(dimethylaminopropyl) carbodiimide |
inactivation by 1-ethyl-3-(dimethylaminopropyl) carbodiimide plus glycine ethyl ester follows a two-phase kinetics: a fast one decreases in presence of melibiose and another slow not affected by melibiose |
|
| 3.2.1.22 | 2,4,6-Trinitrobenzenesulfonic acid |
20% residual activity at 2 mM, at pH 8.4 |
|
