EC Number   |
Inhibitors |
Structure |
|---|
    1.2.7.1 | carbon monoxide |
0.0072 mM, 50% inhibition |
   |
| 1.2.7.1 | dioxygen |
the enzyme is inactivated by dioxygen. Less than 50% of the original activity remains when the protein (0.23 mg/ml in 50 mM Tris/HCl pH 7.5) is exposed to air for 80 min at 25°C. Attempts to reactivate the enzyme by anoxic incubation in the presence of 2 mM dithiothreitol fail |
|
| 1.2.7.1 | Dithionite |
with 5 mM dithionite, the half-life is 7 h at 2 °C, with about 90% of the original activity being lost after 24 h |
|
| 1.2.7.1 | glyoxylate |
20 mM, about 40% of the original activity is lost. 2 mM glyoxylate inhibits 6% |
|
| 1.2.7.1 | KCl |
0.5 M, 50% inhibition |
|
| 1.2.7.1 | KCl |
1020% inhibition is observed with 50 mM KCl, while with higher concentrations (maximum, 0.6 M), 4665% activation is reached, and further increased concentrations of KCl are inhibitory, enzyme Ape2126/2128 |
|
| 1.2.7.1 | more |
the enzyme is not inhibited by sodium nitrite (50 mM) or carbon monoxide (100% CO as gas phase) |
|
| 1.2.7.1 | more |
no substrate inhibition with CoA up to 0.1 mM. Slightly or not inhibited at all by glyoxylate, nitrite, CO or potential physiological effectors. Not inhibited by CO. Potential affectors such as ATP, ADP, AMP, cAMP, GTP, GDP, GMP, NAD+, NADH, and glyceraldehyde 3-phosphate, at concentrations of 2 mM, do not affect the activity |
|
| 1.2.7.1 | more |
not inhibitory: carbon monoxide, sodium cyanide, sodium fluoride, sodium azide |
|
| 1.2.7.1 | NaCl |
the addition of 0.3 M NaCl causes a significant inhibition (up to 80%) of the activity |
|
