EC Number |
Inhibitors |
Structure |
---|
1.1.1.45 | acetoacetate |
competitive inhibitor |
|
1.1.1.45 | ascorbic acid |
molecular dockings of ascorbate to gulonate-3-dehydrogenase indicated its binding near the co-factor binding site. Docking revealed that ascorbate binding could lead to steric clashes between ascorbate and the co-factor (NADH) |
|
1.1.1.45 | Cibacron blue |
competitive inhibition with respect to NAD+ |
|
1.1.1.45 | iodoacetate |
- |
|
1.1.1.45 | malonate |
reversible inhibition, IC50: 0.34 mM |
|
1.1.1.45 | malonic acid monoethyl ester |
weak inhibition, IC50: 5.5 mM |
|
1.1.1.45 | more |
not inhibitory iodoacetate, ethylendiamine tetraacetate, 2,2'-bipyridine, 8-hydroxyquiniline, O-phenanthroline, diethyldithiocarbamate, thiamine pyrophosphate |
|
1.1.1.45 | more |
no inhibition by 20 mM D-glucose, 2 mM 2-keto-L-gulonate, 1 mM D-glucorunate or 6 mM ADP, ATP and AMP; no significant inhibition (less than 15%) by 1 mM L-ascorbate, monocarboxylic acids, or dicarboxylic acids |
|
1.1.1.45 | NADH |
competitive inhibitor |
|
1.1.1.45 | p-chloromercuribenzoate |
in both directions |
|