EC Number   |
General Stability |
Reference |
|---|
   6.1.1.14 | aminoethyl-AMP protects from heat inactivation |
146 |
| 6.1.1.14 | ATP and nonsubstrate ATP analogues render the enzyme more resistant to digestion by several proteases, e.g. thrombin, Arg-C, and chymotrypsin |
650213 |
| 6.1.1.14 | DTT or 25% glycerol stabilizes |
158 |
| 6.1.1.14 | glycerol required for stabilization |
146 |
| 6.1.1.14 | glycyl-adenylate stabilizes the enzyme |
651043 |
| 6.1.1.14 | in absence of protease inhibitors and/or dithioerythritol, the enzyme rapidly loses its activity |
150 |
| 6.1.1.14 | protectors of SH groups at concentrations of 20 mM are required to obtain an optimal aminoacylation rate |
150 |
| 6.1.1.14 | sensitive to repeated freezing and thawing |
156 |
| 6.1.1.14 | unstable after dilution to less than 0.02 mg/ml in absence of nonspecific carrier proteins |
156 |
