EC Number   |
General Stability |
Reference |
|---|
    4.3.2.2 | dialysis for 24 h at 5°C, activity in mutant lysates is decreased by 95% while 50-60% of the initial activity remains in the normal cell lysates |
34462 |
| 4.3.2.2 | freezing at -80°C, followed by thawing, 20-40% loss of enzyme activity in extract |
34466 |
| 4.3.2.2 | stable to thawing and refreezing at a protein concentration of above 2 mg/ml |
34449 |
| 4.3.2.2 | the structure of adenylosuccinate lyase reveals that this protein contains three disulfide bonds that contribute significantly to its stability against thermal and chemical denaturation |
728121 |
| 4.3.2.2 | three cycles of freezing and thawing remove nearly all enzyme activity |
34456 |
| 4.3.2.2 | unstable to quick freezing in dry ice/acetone |
1541 |
