EC Number |
General Stability |
Reference |
---|
4.2.1.33 | 10% inactivation at 0°C in phosphate buffer at pH 7 or above |
5661 |
4.2.1.33 | half-life 2-3 h in potassium phosphate buffer, ph 6.8., 0-5°C, beta-isopropylmalate at 1 mM increases half-life to 15 h |
5663 |
4.2.1.33 | loss of activity after repeated freezing and thawing |
5658, 5661 |
4.2.1.33 | stabilized by high concentrations of glycerol and ammonium sulfate which also inhibit activity, half-life with 50% glycerol, 2.07 M ammonium sulfate, and 30% glycerol plus 830 mM ammonium sulfate: 50 h, 57 h, and 90 h, respectively, instead of 2-3 h without, stabilization probably due to changes in intramolecular interactions |
5659, 5663 |
4.2.1.33 | substrate stabilizes |
5658 |
4.2.1.33 | unstable in phosphate buffer at pH 7.0, 10% loss of activity per h at 0°C |
5658 |