EC Number   |
General Stability |
Reference |
|---|
    2.7.2.2 | 2-mercaptoethanol protects against heat denaturation |
642219 |
| 2.7.2.2 | 80% loss of activity on dialysis against 0.04 M Tris, pH 8.5, 4°C, 18 h, Streptococcus lactis enzyme |
642219 |
| 2.7.2.2 | all the component activities associated with putrescine synthase are stabilized in dilute solutions, 0.05 mg of protein per ml, for about 3-4 h at 37°C by 0.250 mg/ml bovine serum albumin |
209399 |
| 2.7.2.2 | ammonium sulfate, 0.5 M, stabilizes against inactivation |
642219 |
| 2.7.2.2 | DTT is required to prevent the protein from aggregation |
739466 |
| 2.7.2.2 | prolonged dialysis and freeze-thawing, loss of activity |
209399 |
| 2.7.2.2 | purified enzyme is highly unstable even in presence of glycerol, dithiothreitol and Mg2+ |
209399 |
| 2.7.2.2 | unstable in dilute solutions, even at very low temperatures |
642219 |
