EC Number   |
Activating Compound |
Reference |
|---|
    4.2.1.34 | 2,3-dimercapto-1-propanol |
about half as effective as L-Cys |
5668 |
| 4.2.1.34 | 2-mercaptoethylamine |
at pH 8.2-8.4 as effective as L-Cys |
5668 |
| 4.2.1.34 | acetate |
activity is higher in extracts of cells grown on acetate |
705588 |
| 4.2.1.34 | D-Cys |
at pH 8.2-8.4 as effective as L-Cys |
5668 |
| 4.2.1.34 | DL-homocysteine |
at pH 8.2-8.4 as effective as L-Cys |
5668 |
| 4.2.1.34 | L-Cys |
required. The optimal concentration of L-Cys is 0.5-2.0 mM |
5668 |
| 4.2.1.34 | sulfhydryl compounds |
required |
5665, 5666, 5668 |
| 4.2.1.34 | thiol |
aerobic purification results in inactive protein, which can only partly be reactivated with Fe2+ and thiol |
749072 |
| 4.2.1.34 | thiol |
storage of the protein for 6 months leads to almost complete loss of its activity, which can be fully restored by the reactivation with Fe2+ and thiol |
746828 |
