EC Number |
Activating Compound |
Reference |
---|
3.6.4.6 | Ist1 |
addition of the endosomal sorting complex required for the transport III (ESCRT-III) subunit binding partner of Ist1, Did2, converts ESCRT-III subunit Ist1 from an inhibitor to a stimulator of the enzyme (maximal stimulation at 0.008 or less mM). Mutants Ist1(L168A/Y172A) and Ist1(K135A) hyperstimulate enzyme ATPase activity |
757152 |
3.6.4.6 | LIP5 |
- |
689461 |
3.6.4.6 | LIP5F388A |
a weak SKD1 interactor, complements the lip5 mutant for heat and salt stress tolerance, most likely due to its overexpression driven by the strong CaMV 35S promoter in the transgenic plants |
734975 |
3.6.4.6 | Lyst-interacting protein5 |
LIP5, interacts strongly with enzyme SKD1 and stimulates the ATPase activity of the enzyme by 4 to 5fold. LIP5 is a positive regulator of enzyme SKD1 in multivesicular bodies biogenesis and is a critical target of the mitogen-activated protein kinases MPK3 and MPK6 and plays an important role in the plant immune system. Disruption of LIP5 causes compromised tolerance to both heat and salt stresses. When compared with wild-type plants, lip5 mutants accumulate increased levels of ubiquitinated protein aggregates and NaCl under heat and salt stresses, respectively. LIP5 is regulated by phosphorylation, overview. Mutation of MPK phosphorylation sites in LIP5 does not affect its interaction with SKD1 but reduces its stability and, as a result, compromises its ability to complement the basal resistance of the lip5 mutants |
734975 |
3.6.4.6 | more |
activity is dependent upon hexamerisation |
688690 |
3.6.4.6 | more |
binding of substrates to the central pore of the Vps4 ATPase is activated by microtubule interacting and trafficking interacting motifs (MIMs) |
734303 |
3.6.4.6 | more |
Ist1, Did2, Vta1 and Vps60 function as a regulatory system that ensures proper recruitment and assembly of ATPase Vps4 on the ESCRT-III protein complex. Directly or indirectly these factors affect ATPase activity of Vps4, suggesting that they also regulate the Vps4 ESCRT-III disassembly activity |
724447 |
3.6.4.6 | more |
SKD1 E Vps proteins SBP1 and mVps2 interact with SKD1 |
656346 |
3.6.4.6 | more |
the protein VCIP135 is essential for p97/p47-mediated membrane fusion |
656341 |
3.6.4.6 | more |
Vps4p directly binds Vps20p and Vta1p in vitro |
656345 |