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EC Number
Activating Compound
Commentary
Reference
3.4.22.37
2-mercaptoethanol
-
638849
3.4.22.37
2-mercaptoethanol
weak activation, a concentration above 100 mM is required
651891
3.4.22.37
cysteamine
activates, best above 5 mM
651891
3.4.22.37
cysteine
-
638849
3.4.22.37
cysteine
10 mM, most effective reducing agent for activation of the enzyme, denaturation occurs at higher concentration above 5 mM
651891
3.4.22.37
cysteine
activates
669799
3.4.22.37
cysteine
active in presence of
30352
3.4.22.37
cysteine
gingipains are activated by diluting to 10 mM in 0.2 M HEPES, pH 8.0, 5 mM CaCl2 and 10 mM cysteine, and incubation at 37°C for 10 min
699480
3.4.22.37
cysteine
required for full amidolytic activity
30348
3.4.22.37
dithiothreitol
-
638849
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