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EC Number
Activating Compound
Commentary
Reference
3.2.1.11
auxin
-
135737
3.2.1.11
Ca2+
-
135745
3.2.1.11
Co2+
-
135731
3.2.1.11
CoCl2
1 mM, 101% activity compared to control without any metal ion
671015
3.2.1.11
Cu2+
-
135731
3.2.1.11
CuCl2
1 mM, 107% activity compared to control without any metal ion
671015
3.2.1.11
Mg2+
-
135745
3.2.1.11
Mn2+
-
135731
,
135745
3.2.1.11
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bentonite carriers exhibit high monolayer protein and activity loads at pH 4.0 and after pH-shift to 5.4. If dextranase is adsorbed at pH 4.1 and hydroxyapatite is washed several times with buffer at pH 5.4 reveals highest activity yield so far (28%). Other selected carriers used are hydroxyapatite and Streamline DEAE. In general, immobilization yields are low and in case of DEAE the rather high particle size is problematic. Porous Eupergit differs only moderately in activity using low molecular weight substrate, whereas macroporous Eupergit reveals enhanced activity for high molecular weight substrates. Activation of the carbohydrate moiety of dextranase by metaperiodate results in active silica gels, whereas the activation of alginate via Woodwards reagent K exhibits extremely low activities
680987
3.2.1.11
more
highest immobilization efficiency at 200 mg Eupergit C with a ratio of activity of immobilized enzyme to the activity of soluble enzyme of 90%, higher amounts of Eupergit C yield lower immobilization efficiency. Immobilization duration increases immobilization efficiency
678011
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