EC Number |
Activating Compound |
Reference |
---|
1.8.1.4 | cysteine |
NADH-oxidation with free lipoic acid is strongly dependent on the addition of NAD+, EDTA, Mg2+ and cysteine, the reverse reaction with reduced lipoic acid and NAD+ does not show any requirement for cofactors |
394001 |
1.8.1.4 | dithiothreitol |
presence during purification preserves enzymatic activity |
725303 |
1.8.1.4 | EDTA |
NADH-oxidation with free lipoic acid is strongly dependent on the addition of NAD+, EDTA, Mg2+ and cysteine, the reverse reaction with reduced lipoic acid and NAD+ does not show any requirement for cofactors |
394001 |
1.8.1.4 | Guanidine-HCl |
4°C: activates the enzyme 2.5fold at 0.2 M |
659611 |
1.8.1.4 | KCl |
4°C: activates the enzyme at concentrations below 1 M |
659611 |
1.8.1.4 | lipoic acid |
increase in activity might be due to formation of dihydrolipoic acid in the assay system |
658431 |
1.8.1.4 | methylene blue |
stimulates reduction of cytochrome c and myoglobin |
658742 |
1.8.1.4 | more |
activation of DLD proteolytic activity by high salt concentrations during hydroxyapatite fractionation, Freezingthawing results in further activation |
694854 |
1.8.1.4 | more |
activation of DLD proteolytic activity by high salt concentrations during hydroxyapatite fractionation. Freezing-thawing results in further activation |
694854 |
1.8.1.4 | more |
activity of DLDH dehydrogenase increases in rats between 10 and 20 days of age. Dehydrogenase activity of DLDH shows a further, progressive increase in rats from 20 days to adulthood, in the absence of any further change in DLDH expression or diaphorase activity |
693975 |