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EC Number
Activating Compound
Commentary
Reference
1.3.1.14
2-mercaptoethanol
activates
390568
1.3.1.14
cysteine
essential for assay, interaction of enzyme with orotate or dihydroorotate is dependent on cysteine, whereas NADH oxidase activity is not
390561
,
390566
1.3.1.14
cysteine
L-cysteine activates strongly, D-cysteine is also an activator, 1.8 mM L-cysteine is required at pH 8 for half-maximal activity, 10 mM for near-maximal activity
390568
1.3.1.14
cysteine
reaction rate increases 2fold in the presence of 2-7 mM cysteine
390562
1.3.1.14
cysteine
required for activation
390564
1.3.1.14
cysteine
required for activation of purified enzyme, but not if crude extract is used for assay
390566
1.3.1.14
dithiothreitol
activates
390568
1.3.1.14
glutathione
activates
390568
1.3.1.14
glutathione
stimulating effect, but less effective than cysteine
390561
1.3.1.14
Mercaptoacetic acid
activates
390568
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