EC Number   |
Activating Compound |
Reference |
|---|
   1.13.12.5 | (1,8-bis-maleimidotriethylene glycol) |
effective conjugation of the N-terminal and C-terminal fragments of Rluc, results in enzymatic activity |
696498 |
| 1.13.12.5 | ATP |
50 microM |
695572 |
| 1.13.12.5 | bovine serum albumin |
addition to reaction mixture enhances luciferase activity |
439334 |
| 1.13.12.5 | CaCl2 |
0.1 mM |
695982 |
| 1.13.12.5 | coelenterazine |
increase of wild-type Gluc activity at higher substrate concentrations |
695563 |
| 1.13.12.5 | histamine |
10 microM |
695572 |
| 1.13.12.5 | KCl |
substitution of NaCl by KCl does not significantly alter the dependence of light intensity on salt concentrations |
765264 |
| 1.13.12.5 | more |
Ca2+-induced interaction between calmodulin and M13 leads to intermolecular complementation of split Renilla luciferase |
695572 |
| 1.13.12.5 | more |
complexation of GLuc with streptavidin after biotinylation of GLuc |
695558 |
| 1.13.12.5 | more |
hybridization of two oligonucleotide probes with fused Rluc fragments resulted in reassembly of the fragment, generating active Rluc |
696498 |
