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1.1.1.268
ethanesulfonate
1 mM, 5.6fold increase in ratio of turnover number to Km-value for 2-butanone as substrate
654760
1.1.1.268
ethanesulfonate
the addition of ethanesulfonate dramatically increases the enentiomeric excess of (S)-2-butanol produced by all forms of recombinant R-HPCDH except the R152A and R196A mutants. The effect of ethanesulfonate and other additives on (S)-butanol production is termed enantioselective modulation, and it is highly selective for sulfonate containing molecules. The interpretation is that the sulfonate of ethanesulfonate interacts with R152 and R196 in the CoM binding pocket alongside 2-butanone, in a way that discourages a si-face hydride addition to produce (R)-2-butanol
762714
1.1.1.268
methanesulfonate
1 mM, 5.6fold increase in ratio of turnover number to Km-value for 2-butanone as substrate
654760
1.1.1.268
propanesulfonate
1 mM, 1.7fold increase in ratio of turnover number to Km-value for 2-butanone as substrate
654760
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