EC Number |
Activating Compound |
Reference |
---|
2.7.8.8 | cardiolipin |
activates. The enzyme is completely desensitized by treatment for 5 min at 40°C against the effect of cadiolipin without loss of activity |
643784 |
2.7.8.8 | diphosphatidylglycerol |
membrane association and activity of PtdSer synthase is increased, studied with mixed micelles containing phosphatidylglycerol (one charge) or diphosphatidylglycerol (two charges), the two main anionic membrane lipids in Escherichia coli |
661745 |
2.7.8.8 | more |
activity of phosphatidylserine synthase depends significantly on the nature and level of the lipids in the matrix, at which the enzyme is operating |
661745 |
2.7.8.8 | more |
optimal activity is dependent on ionic strength, 0.3 or higher |
643778 |
2.7.8.8 | more |
the enzyme reconstituted with lipid vesicles of various compositions exhibits practically no activity in the absence of a detergent and with the substrate CDP-diacylglycerol present only in the lipid vesicles. Inclusion of octylglucoside in the assay mixture increases the activity 20- to 1000fold, the degree of activation depends on the lipid composition of the vesicles. Inclusion of additional CDP-diacylglycerol in the assay mixture increases the activity 5- to 25-fold. When the fraction of phosphatidylglycerol is increased from 15 to 100 mol% in the vesicles the activity increases 10fold using the assay mixture containing octylglucoside. The highest activities are exhibited with the anionic lipids diphosphatidylglycerol and phosphatidic acid while phosphatidylinositol gives lower activity |
643796 |
2.7.8.8 | phosphatidylethanolamine |
slightly activates. The enzyme is completely desensitized by treatment for 5 min at 40°C against the effect of phosphatidylethanolamine without loss of activity |
643784 |
2.7.8.8 | phosphatidylglycerol |
membrane association and activity of PtdSer synthase is increased, studied with mixed micelles containing phosphatidylglycerol (one charge) or diphosphatidylglycerol (two charges), the two main anionic membrane lipids in Escherichia coli |
661745 |
2.7.8.8 | Triton X-100 |
dependent on nonionic detergent, at 0.1 mM CDP-diacylglycerol optimal activity occurs at a Triton to substrate molar ratio of 8:1 |
643779 |
2.7.8.8 | Triton X-100 |
enzyme is dependent on a nonionic detergent such as Triton X-100 |
643778 |
2.7.8.8 | Triton X-100 |
increasing levels of Triton X-100 at low molecular ratios of Triton X-100 to CDP-diacylglycerol stimulate |
643782 |