6.3.3.2	D124A	inactive	728751	
6.3.3.2	D151A	the mutant shows increased turnover number compared to the wild type enzyme	728751	
6.3.3.2	D154A	inactive	728751	
6.3.3.2	D154A	site-directed mutagenesis, the mutant shows 0.9% of the wild-type catalytic activity	703027	
6.3.3.2	D81A	the mutant shows reduced turnover number compared to the wild type enzyme	728751	
6.3.3.2	E225Stop	mutation identified in a patient suffering MTHFD1 deficiency	745499	
6.3.3.2	E61A	site-directed mutagenesis, the mutant shows 8.6% of the wild-type catalytic activity	703027	
6.3.3.2	F55A	site-directed mutagenesis, the mutant shows 53.6% of the wild-type catalytic activity	703027	
6.3.3.2	F85A	site-directed mutagenesis, the mutant shows 72.3% of the wild-type catalytic activity	703027	
6.3.3.2	K10A	site-directed mutagenesis, the mutant shows 7.5% of the wild-type catalytic activity	703027