6.1.1.18	A29X	site-directed mutagenesis	716940	
6.1.1.18	C229R	site-directed mutagenesis, transplanting the conserved arginine residue from glutamyl-tRNA synthetase, EC 6.1.1.17, to glutaminyltRNA synthetase improves the KM of GlnRS for noncognate glutamate	694903	
6.1.1.18	C229R/Q255I	site-directed mutagenesis, comparison of mutant activity with glutamate and glutamine to charge tRNAGln to the wild-type activity, the mutant shows no activity with L-Gln, but weakly with L-Glu	694903	
6.1.1.18	C229R/Q255I/S227A/F233Y	site-directed mutagenesis, comparison of mutant activity with glutamate and glutamine to charge tRNAGln to the wild-type activity, the mutant shows no activity with L-Gln, but activity with L-Glu	694903	
6.1.1.18	cGluGlnRS	a chimeric protein, consisting of the catalytic domain of GluRS and the anticodon-binding domain of GlnRS, is constructed	703714	
6.1.1.18	D235A	saturation mutagenesis, only little complementation of glnS-deficient strain	649850	
6.1.1.18	D486R/L488Q	the double mutant causes a relaxed tRNA anticodon specificity	726984	
6.1.1.18	D66E	saturation mutagenesis, 18fold increased Km for glutamine, decreased turnover	649850	
6.1.1.18	D66F	saturation mutagenesis, highly increased Km for glutamine, 1200fold decrease in activity	649850	
6.1.1.18	D66G	saturation mutagenesis, only little complementation of glnS-deficient strain	649850