4.2.1.30 I498A optimal pH and optimal temperature as the wild-type. KM for glycerol comparable to the wild-type -, 704770 4.2.1.30 I498C active, optimal pH as the wild-type, lower optimal temperature as the wild-type. KM for glycerol comparable to the wild-type -, 704770 4.2.1.30 I498D active, lower optimal pH as the wild-type, optimal temperature as the wild-type. Higher KM for glycerol as the wild-type -, 704770 4.2.1.30 I498M active, lower optimal pH and lower optimal temperature as the wild-type. Lower KM for glycerol as the wild-type -, 704770 4.2.1.30 I498N active, optimal pH as the wild-type, lower optimal temperature as the wild-type. Lower KM for glycerol as the wild-type 704770 4.2.1.30 I498P active, lower optimal pH and lower optimal temperature as the wild-type. Higher KM for glycerol as the wild-type 704770 4.2.1.30 I498S active, lower optimal pH and lower optimal temperature as the wild-type. KM for glycerol comparable to the wild-type 704770 4.2.1.30 I498T active, lower optimal pH as the wild-type, optimal temperature as the wild-type. Lower KM for glycerol as the wild-type 704770 4.2.1.30 I498V alpha subunit has half-life of 11 h in contrast to 1 h for the wild-type. Optimal pH and optimal temperature as the wild-type. KM for glycerol comparable to the wild-type 704770 4.2.1.30 I498V/Q42L alpha subunit of I498V and beta subunit of Q42L have half-life of 16 h in contrast to 1 h for the wild-type 704770