4.2.1.134 E116A phytosphingosine accumulates at low levels in the mutant compared with wild type cells 719847 4.2.1.134 E156A inactive, the mutant cannot suppress phytosphingosine accumulation, although the protein is expressed normally 719847 4.2.1.134 E60A phytosphingosine accumulates at low levels in the mutant compared with wild type cells 719847 4.2.1.134 F145A phytosphingosine accumulates at low levels in the mutant compared with wild type cells 719847 4.2.1.134 G152A mutant with reduced activity, the mutant cannot suppress phytosphingosine accumulation, although the protein is expressed normally 719847 4.2.1.134 L12A phytosphingosine accumulates at low levels in the mutant compared with wild type cells 719847 4.2.1.134 additional information construction of a Sporobolomyces phs1 mutant strain GI209 by T-DNA insertion. The mutant has less dehydratase activity and a reduction in very long chain fatty acids compared to wild-type. The phs1 mutation in Sporobolomyces causes reduction in 3-hydroxyacyl-CoA dehydratase activity and alters the lipid profiles of the cells. The mutant strain also exhibits sensitivity to cell wall stress agents and loss of shooting due to a delay in ballistospore formation -, 749134 4.2.1.134 additional information construction of an enzyme deletion mutant DELTAPH, phenotype, overview -, 748533 4.2.1.134 N16A phytosphingosine accumulates at low levels in the mutant compared with wild type cells 719847 4.2.1.134 P150A phytosphingosine accumulates at low levels in the mutant compared with wild type cells 719847