4.2.1.112	C141S	site-directed mutagenesis	748243, 748431	
4.2.1.112	D13A	site-directed mutagenesis of catalytically important Asp13, a direct neighbor of the [4Fe-4S] coordinating Cys12, forms a close hydrogen bond of 2.41 A to the oxygen ligand of the W ion, the mutant shows significant loss of activity compared to wild-type	748243, 748431	
4.2.1.112	D13E	site-directed mutagenesis of catalytically important Asp13, a direct neighbor of the [4Fe-4S] coordinating Cys12, forms a close hydrogen bond of 2.41 A to the oxygen ligand of the W ion, the mutant shows unaltered activity compared to wild-type	748243, 748431	
4.2.1.112	D13E	site-directed mutagenesis, almost inactive mutant	-, 715374	
4.2.1.112	I142A	site-directed mutagenesis, Ile142 is part of the hydrophobic ring that is proposed to form the substrate binding cavity at the end of the access tunnel towards the active site, its exchange against alanine results in a strong loss of activity	748243, 748431	
4.2.1.112	I142A	site-directed mutagenesis, the mutant shows a marked loss of activity compared to the wild-type enzyme	-, 715374	
4.2.1.112	K48A	site-directed mutagenesis of the residue involved in electron transfer between the two cofactors, the exchange of Lys48 against alanine does not affect catalysis	748243, 748431	
4.2.1.112	K48A	site-directed mutagenesis, the mutant shows unaltered activity compared to the wild-type enzyme	-, 715374	
4.2.1.112	additional information	construction of active-site variants, and of a fusion protein of the N-terminal chaperone binding site of the Escherichia coli nitrate reductase NarG to the AH gene improving the yield and activity of AH and its variants significantly, overview	-, 715374