3.5.1.89	D102A	site-directed mutagenesis of the isolated cytoplasmic catalytic domain, the mutant is active in absence of metal ions, but well stimulated by metal ions	734251	
3.5.1.89	D133A	site-directed mutagenesis of the isolated cytoplasmic catalytic domain, the mutant is active in absence of metal ions, but well stimulated by metal ions	734251	
3.5.1.89	D45A	site-directed mutagenesis of the isolated cytoplasmic catalytic domain, the mutant is active in absence of metal ions, but well stimulated by metal ions	734251	
3.5.1.89	D46A	site-directed mutagenesis of the isolated cytoplasmic catalytic domain, the mutant is only slightly active in absence of metal ions and not stimulated by metal ions	734251	
3.5.1.89	D47A	site-directed mutagenesis of the isolated cytoplasmic catalytic domain, the mutant is only slightly active in absence of metal ions, but well stimulated by metal ions	734251	
3.5.1.89	E79A	site-directed mutagenesis of the isolated cytoplasmic catalytic domain, the mutant is active in absence of metal ions, but well stimulated by metal ions	734251	
3.5.1.89	H140A	site-directed mutagenesis of the isolated cytoplasmic catalytic domain, the mutant is only slightly active in absence of metal ions and not stimulated by metal ions	734251	
3.5.1.89	H143A	site-directed mutagenesis of the isolated cytoplasmic catalytic domain, the mutant is only slightly active in absence of metal ions, but well stimulated by metal ions	734251	
3.5.1.89	H43A	site-directed mutagenesis of the isolated cytoplasmic catalytic domain, the mutant is only slightly active in absence of metal ions, but well stimulated by metal ions	734251	
3.5.1.89	additional information	a CaGPI12 heterozygous is generated by disrupting one allele with HIS1 by a PCR-mediated approach using CaGPI12-HIS1 FP and CaGPI12-HIS1 RP by the lithium-acetate (LiAc) method. The CaGPI12 conditional null mutant is made by replacing its native promoter with MET3 promoter by using the pMET3-GFP-URA3 cassette. CaGPI12 is able to rescue the growth defect of the ScGPI12 conditional null strain as compared to the control strain carrying the YepHIS vector alone, suggesting that CaGPI12 functionally complements ScGPI12. Phenotype CaGPI12 and ScGPI12 conditional null strains, overview	-, 756746