3.4.21.64	A199S	no effect on activity	678927	
3.4.21.64	A236V	lethal	678927	
3.4.21.64	E138A	lethal, increased stability of the serine protease subtilisin	678927	
3.4.21.64	G293A	strong positive effect on activity	678927	
3.4.21.64	I132V	positive effect on activity	678927	
3.4.21.64	I310K	no effect on activity, increased stability of the serine protease subtilisin	678927	
3.4.21.64	K208H	positive effect on activity	678927	
3.4.21.64	K332R	positive effect on activity	678927	
3.4.21.64	L180I	positive effect on activity	678927	
3.4.21.64	L299C	lethal, increased stability of the serine protease subtilisin	678927