3.2.2.30 D198N inactive 727008, 752041 3.2.2.30 D199A active-site mutant 726595 3.2.2.30 D199A inactive 726595 3.2.2.30 D199N active-site mutant 726595 3.2.2.30 D199N the mutant shows residual activity 726595 3.2.2.30 E14Q active-site mutant 726595 3.2.2.30 E14Q the mutant shows residual activity 726595 3.2.2.30 E173Q inactive 727044 3.2.2.30 F104C/C186S the mutations give an enzyme with steady-state kinetic properties similar to the native enzyme 727044