3.2.1.57	D353N	enzyme shows no activity with pullulan and panose as substrate	664832	
3.2.1.57	D372N	enzyme shows no activity with pullulan and panose as substrate	664832	
3.2.1.57	D373N	enzyme shows no activity with pullulan and panose as substrate	664832	
3.2.1.57	E273N	45% of wild-type activity with pullulan as substrate, 74% of wild-type activity with panose as substrate	664832	
3.2.1.57	E356Q	38% of wild-type activity with pullulan as substrate, 50% of wild-type activity with panose as substrate	664832	
3.2.1.57	N448A	site-directed mutagenesis, glycan-deficient variant, Asn448 is not N-glycosylated, the mutant shows reduced thermal stability compared to the wild-type enzyme	-, 732013	
3.2.1.57	S450A	site-directed mutagenesis, glycan-deficient variant, by replacing Ser450 with Ala, Asn448 is not N-glycosylated, but the Asn448 side chain remains intact, unlike the Asn448Ala mutation, the mutant shows reduced thermal stability compared to the wild-type enzyme	-, 732013	
3.2.1.57	W240F	130% of wild-type activity with pullulan as substrate, 160% of wild-type activity with panose as substrate	664832	
3.2.1.57	W31F	38% of wild-type activity with pullulan as substrate, 140% of wild-type activity with panose as substrate	664832	
3.2.1.57	W32F	90% of wild-type activity with pullulan as substrate, 120% of wild-type activity with panose as substrate	664832