2.6.1.27 additional information an auxin-defective mutant, tillering and small grain 1 (tsg1), displays an increased number of tillers but reduced panicle and grain size due to mutation of TSG1, which encodes a tryptophan aminotransferase. TSG1 is allelic to the previously identified FISH BONE (FIB) gene, loss-of-function which results in pleiotropic abnormal phenotypes. The TSG1 gene, an F2 population derived from a cross between the tsg1 mutant line and japonica rice variety Zhonghua-11 (ZH11), is obtained using the map-based cloning approach. The TSG1 locus is originally mapped to the long arm of chromosome 1 between marker loci G01856 and G011812 using 636 homozygous F2 plants then subsequently fine-mapped to a 26-kb region between marker loci G011422 and G011436, which contains two open reading frames (ORFs), LOC_Os01g07490 and LOC_Os01g07500. Knockout of TSG1 using clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) genome editing in ZH11-produced plants reveal a phenotype similar to that of the natural tsg1 mutant. Phenotypes, overview 759589 2.6.1.27 additional information in isoform tam1 deletion mutant, pigment production is severely affected, and mutants produced fewer pigment compounds than the wild type 689054