2.3.1.255 E24A mutation in NatA, decrease in kcat, increase in Km value 736866 2.3.1.255 E24D mutation in NatA, decrease in kcat, increase in Km value 736866 2.3.1.255 E24Q mutation in NatA, decrease in kcat, increase in Km value 736866 2.3.1.255 E61A mutation in NatA, decrease in kcat, increase in Km value 736866 2.3.1.255 E62A mutation in NatA, decrease in kcat, increase in Km value 736866 2.3.1.255 F128I site-directed mutagenesis, the mutation leads an altered structure and reduced stability, and a dramatic recuction of Nt catalytic activity compared to wild-type 756675 2.3.1.255 F128L site-directed mutagenesis, the mutation leads an altered structure and reduced stability, and a dramatic recuction of Nt catalytic activity compared to wild-type 756675 2.3.1.255 H111A mutation in NatA, decrease in kcat, Km value similar to wild-type 736866 2.3.1.255 H20A mutation in NatA, decrease in kcat, increase in Km value 736866 2.3.1.255 H72A mutation in NatA, decrease in kcat, increase in Km value 736866