1.20.99.1	C120A	inactive mutant	714217	
1.20.99.1	C71S	mutagenesis of cysteine residues in the putative active site HC(X) 5 R motif leads to nearly complete loss of both phosphatase and arsenate reductase activities	694407	
1.20.99.1	additional information	catalytic domain of Cdc25B is expressed in bacteria. Cdc25B shows arsenate reductase activity. Enzyme reduces inorganic arsenate but not methylated pentavalent arsenicals. Alteration of either the cysteine and arginine residues of the Cys-X5-Arg motif leads to the loss of both reductase and phosphatase activities	714217	
1.20.99.1	R126A	inactive mutant	714217	
1.20.99.1	R60A	site-directed muatgenesis, crystal structure determination and analysis with bound arsenite, formation of a covalent and more stableCys12-thiol-dihydroxyarsenite intermediate compared to the wild-type enzyme, resulting in slow release of product and loss of activity	660461	
1.20.99.1	R60K	site-directed muatgenesis, crystal structure determination and analysis with bound arsenite, formation of a covalent and more stableCys12-thiol-dihydroxyarsenite intermediate compared to the wild-type enzyme, resulting in slow release of product and loss of activity	660461