1.2.4.4	D108N	less thermostable than wild type enzyme	687543	
1.2.4.4	D200A	less thermostable than wild type enzyme	687543	
1.2.4.4	D200A/D108N	the mutant is defective in folding and assembly and as no detectable overall activity	687543	
1.2.4.4	D200N	less thermostable than wild type enzyme	687543	
1.2.4.4	D295A	site-directed mutagenesis of an alpha-subunit residue of component E1b, increased activity compared to the wild-type	656230	
1.2.4.4	D296A	inactive enzyme, no ability of component E1 apoenzyme to reconstitute with thiamine diphosphate	349090	
1.2.4.4	E198Q/D200N	the mutant is defective in folding and assembly and as no detectable overall activity	687543	
1.2.4.4	F364C	E1alpha-missense mutation in type IA maple syrup urine disease that causes the occurence of exclusively alpha,beta dimers	349091	
1.2.4.4	H146A	site-directed mutagenesis, mutation of a beta'-subunit residue, no reductive acylation activity, low decarboxylation activity	656171	
1.2.4.4	H147N	site-directed mutagenesis, mutation of a beta'-subunit residue, no reductive acylation activity, low decarboxylation activity	656171