1.1.1.286	I82N	site-directed mutagenesis, the mutant shows reduced activity and altered substrate specificity compared to the wild-type enzyme	-, 740021	
1.1.1.286	L81P	site-directed mutagenesis, the mutant shows reduced activity and altered substrate specificity compared to the wild-type enzyme	-, 740021	
1.1.1.286	L83R	site-directed mutagenesis, the mutant shows reduced activity and altered substrate specificity compared to the wild-type enzyme	740021	
1.1.1.286	additional information	deletion of A80, inability of mutant to use 3-isopropylmalate, isocitrate is 4fold preferred over homoisocitrate	660918	
1.1.1.286	additional information	generation of a gene-disruption TK0280 deletion mutan strain, functional complementation by expression of the gene icdh from Thermus thermophilus strain Hb27, but not by genes hicdh and leuB from this organism	-, 740021	
1.1.1.286	R85V	complete loss of activity with citrate, retains activity with isocitrate	-, 656127	
1.1.1.286	R85V	increased ability to use 3-isopropylmalate	660918	
1.1.1.286	R87T	uses substrate homoisocitrate, but not substrates isocitrate or 3-isopropylmalate	660918	
1.1.1.286	R87V	uses substrate homoisocitrate, but not substrates isocitrate or 3-isopropylmalate	660918	
1.1.1.286	S80A	site-directed mutagenesis, the mutant shows reduced activity and altered substrate specificity compared to the wild-type enzyme	-, 740021