3.5.99.5 additional information spontaneous mutants HS121(pNB2) during serial transfer on nonselective agar plates, can not grow on nitrobenzene as the sole source of carbon, nitrogen and energy, have lost plasmid pNB1, plasmid-cured HS124 mutant, obtained by incubating the parent strain at an elevated temperature, have lost plasmid pNB2 and also can not grow on nitrobenzene -, 669076 3.5.99.5 R56A the mutant loses the ability to maintain the hexameric conformation, and reveales that the hydrogen bonds between residues Arg56 and Met96 have pivotal roles in the AmnE hexameric assembly 777427