3.2.1.B44 C331A the mutant enzyme catalyzes the hydrolysis of glycyrrhizin at less than 5% of the activity compared to the wild-type enzyme -, 749672 3.2.1.B44 C331D the mutant enzyme catalyzes the hydrolysis of glycyrrhizin at about 25% of the activity compared to the wild-type enzyme 749672 3.2.1.B44 C331H the mutant enzyme catalyzes the hydrolysis of glycyrrhizin at about 30% of the activity compared to the wild-type enzyme 749672 3.2.1.B44 C331K the mutant enzyme catalyzes the hydrolysis of glycyrrhizin at about 25% of the activity compared to the wild-type enzyme 749672 3.2.1.B44 C331L the mutant enzyme catalyzes the hydrolysis of glycyrrhizin at about 65% of the activity compared to the wild-type enzyme 749672 3.2.1.B44 C331S the mutant enzyme catalyzes the hydrolysis of glycyrrhizin at about 30% of the activity compared to the wild-type enzyme -, 749672 3.2.1.B44 H365A the mutant enzyme catalyzes the hydrolysis of glycyrrhizin at about 40% of the activity compared to the wild-type enzyme -, 749672 3.2.1.B44 H365C the mutant enzyme catalyzes the hydrolysis of glycyrrhizin at about 50% of the activity compared to the wild-type enzyme 749672 3.2.1.B44 H365D the mutant enzyme catalyzes the hydrolysis of glycyrrhizin at about 20% of the activity compared to the wild-type enzyme 749672 3.2.1.B44 H365K the mutant enzyme catalyzes the hydrolysis of glycyrrhizin at about 60% of the activity compared to the wild-type enzyme 749672