3.1.3.75 C87A involved in a non-essential intramolecular disulfide bond 706604 3.1.3.75 C94A involved in a non-essential intramolecular disulfide bond 706604 3.1.3.75 D123N site-directed mutagenesis, the mutant enzyme fragment shows reduced activity compared to the wild-type enzyme fragment 664211 3.1.3.75 D203S site-directed mutagenesis, inactive mutant enzyme fragment 664211 3.1.3.75 D262E low PChP activity 679380 3.1.3.75 D262E mutant shows activity less than 2% with respect to native recombinant enzyme 696420 3.1.3.75 D265E enzyme activity reduced to 30-35% of wild-type activity 679380 3.1.3.75 D267E low PChP activity 679380 3.1.3.75 D267E mutant shows activity less than 2% with respect to native recombinant enzyme and is not activated by Cu2+ 696420 3.1.3.75 D31E protein expression, but no enzyme activity 679380