2.9.1.2	A239T	naturally occurring mutation and site-directed mutagenesis. The mutant forms a stable complex with GroEL. Residue Ala239 is located in helix alpha8 near the site that interacts with the variable arm of tRNASec, and is distant from the active site. The A239T variant binds tRNASec with less affinity compared to wild-type SepSecS, but its catalytic function is unaffected	762417	
2.9.1.2	H166A	the mutant is partially active in forming Sec-tRNASec in vivo. In vitro, the mutant is partially active in forming Cys-tRNASec	694428	
2.9.1.2	H166F	mutant is inactive in vivo	694428	
2.9.1.2	H166Q	mutant is inactive in vivo	694428	
2.9.1.2	K173A	in vivo activity of the mutant is indistinguishable from that of the wild-type enzyme	695136	
2.9.1.2	K173M	in vivo activity of the mutant is indistinguishable from that of the wild-type enzyme	695136	
2.9.1.2	additional information	mapping pathogenic mutations onto the tetrameric human SepSecS-tRNASec complex, overview	762417	
2.9.1.2	Q105A	mutant is inactive in vivo	695136	
2.9.1.2	R307A	the mutant is significantly less active in L-selenocysteinyl-tRNASec formation in vivo and Cys-tRNASec formation in vitro	694428	
2.9.1.2	R313A	mutant is inactive in vivo	695136