2.7.1.44 A386S possibly critical role in catalysis, relative activity: 90% (wild-type: 100%) 704516 2.7.1.44 A41E possibly critical for sugar binding, relative activity: 0% (wild-type: 100%) 704516 2.7.1.44 Y250F possibly important for substrate specificity (galacturonic acid /glucuronic acid), relative activity: 90% (wild-type: 100%), 50% activity with D-glucuronic acid (wild-type: 0%) 704516