2.1.1.11 C111S 0.1% of wild-type activity 757980 2.1.1.11 C111S/C115S only residual in vitro activity under reducing conditions, almost complete loss of activity under non-reducing conditions 757980 2.1.1.11 C115S 20% of wild-type activity, strong stimulation by dithiothreitol 757980 2.1.1.11 C115S/C177S only residual in vitro activity under reducing conditions, almost complete loss of activity under non-reducing conditions 757980 2.1.1.11 C177S residue is responsible for redox-dependent enzyme activation. In absence of dithiothreitol, increased activity compared with wild-type. Only slight activation by dithiothreitol 757980 2.1.1.11 H139A loss of more than 80% of methyltransferase activity, mutant has comparable Kd values for Mg-phosphate as the wild type protein 734272 2.1.1.11 H139N loss of more than 80% of methyltransferase activity, mutant has comparable Kd values for Mg-phosphate as the wild type protein 734272 2.1.1.11 H139Q loss of more than 80% of methyltransferase activity, mutant has comparable Kd values for Mg-phosphate as the wild type protein 734272 2.1.1.11 L225F mutation yellow-green leaf 18, leads to yellow-green leaves, decreased chlorophyll level, and climate-dependent growth differences 756823 2.1.1.11 L271P naturally occuring mutation, Mg-protoporphyrin IX accumulated in the mutant, a low activity of the mutant protein is not excluded 720712