1.3.1.20 A36D production by site-directed mutagenesis 684150 1.3.1.20 A36D shows a decreased kcat value compared to the wild type enzyme 684150 1.3.1.20 C145S same sensitivity to SH-reagents as wild-type 349250 1.3.1.20 C170A kinetically similar to wild-type 349257 1.3.1.20 C193S no sensitivity to SH-reagents remaining 349250 1.3.1.20 C217A 4fold increase in Km for 5alpha-androstan-3,17-dione and 2fold increase in Km for NADH 349257 1.3.1.20 C87S DD1 mutant 349261 1.3.1.20 D280A production by site-directed mutagenesis 684150 1.3.1.20 D280A shows a decreased kcat value compared to the wild type enzyme 684150 1.3.1.20 D50N low dehydrogenase activity 349250