1.2.1.100	E149Q	mutant shows a showed a different pH optimum depending on the cosubstrate. With NAD+, the mutant shows very low activity with an optimum pH at 8.5 in the universal buffer. In contrast, the optimum pH is 5.5 with NADH	-, 745236	
1.2.1.100	H137L	almost complete loss of activity	-, 745236