4.1.1.64 additional information gene knockout mutants display wild-type morphology and pathogenicity, can use alanine as sole nitrogen source, but not 2-methylalanine 4.1.1.64 Q52A 85fold decrease in turnover number for decarboxylation half-reaction with 2-aminoisobutyrate and pyruvate, greatly reduced rate of transamination half-reaction and impaired pyridoxamine phosphate binding 4.1.1.64 Q52E 10000fold decrease in turnover number for decarboxylation half-reaction with 2-aminoisobutyrate and pyruvate, transamination rate is nearly identical to wild-type value 4.1.1.64 Q52I 100000fold decrease in turnover number for decarboxylation half-reaction with 2-aminoisobutyrate and pyruvate, greatly reduced rate of transamination half-reaction and impaired pyridoxamine phosphate binding