4.1.1.25 A295F site-directed saturation mutagenesis, the mutant shows reduced activity compared to wild-type 749322 4.1.1.25 E102A site-directed saturation mutagenesis, the mutant shows reduced activity compared to wild-type 749322 4.1.1.25 E299A site-directed saturation mutagenesis, the mutant shows reduced activity compared to wild-type 749322 4.1.1.25 F338Y alteration in the primary activity from decarboxylation/deamination to decarboxylation. Mutant displays a very low activity to tyrosine, i.e. about 5% of its activity to phenylalanine, and strong activity to DOPA 727952 4.1.1.25 F346Y alteration in the primary activity from decarboxylation-deamination to decarboxylation. Mutant retains a small percentage of decarboxylation-deamination activity 727952 4.1.1.25 G296F site-directed saturation mutagenesis, the mutant shows reduced activity compared to wild-type 749322 4.1.1.25 H241N site-directed saturation mutagenesis, almost inactive mutant 749322 4.1.1.25 H241Q site-directed saturation mutagenesis, almost inactive mutant 749322 4.1.1.25 H391A site-directed saturation mutagenesis, the mutant shows reduced activity compared to wild-type 749322 4.1.1.25 H98A site-directed saturation mutagenesis, the mutant shows reduced activity compared to wild-type 749322 4.1.1.25 K240A site-directed saturation mutagenesis, the mutant shows 36% reduced catalytic efficiency compared to wild-type 749322 4.1.1.25 M505A site-directed saturation mutagenesis, the mutant shows reduced activity compared to wild-type 749322 4.1.1.25 M588A site-directed saturation mutagenesis, the mutant shows reduced activity compared to wild-type 749322 4.1.1.25 M99A site-directed saturation mutagenesis, the mutant shows reduced activity compared to wild-type 749322 4.1.1.25 additional information construction of enzyme knockout mutant strain V583 DELTAtdc, a non-tyramine-producing mutant that lacks the decarboxylase genes cluster. Gene expression of aguA gene is measured by quantitative RT-PCR in cultures of the wild-type and DELTAtdc knockout mutant strains -, 747848 4.1.1.25 additional information fusion construct of tyrosine decarboxylase and tyramine N-hydroycinnamoyltransferase 702831 4.1.1.25 additional information generation of a chimeric protein composed of Thalictrum flavum tyrosine decarboxylase residues 1–300 and Petroselinum crispum romatic acetaldehyde synthase residues 300–514. The hybrid enzyme behaves primarily as a wild-type Petroselinum crispum acetaldehyde synthase 727952 4.1.1.25 additional information structure-guided site-directed mutagenesis and alanine scanning and saturation mutagenesis of LbTDC are performed on residues around the substrate binding sites and those required for conformational stability to elucidate the function of key residues involved in the catalytic mechanism and to promote the potential applications of LbTDC in tyramine synthesis, food safety, and pharmacology 749322 4.1.1.25 additional information the mutant allele of Tdc1, Tdc1f03311, reduces expression of the mature Tdc1 transcript by greater than 100fold 704860 4.1.1.25 N100A site-directed saturation mutagenesis, the mutant shows reduced activity compared to wild-type 749322 4.1.1.25 P397A site-directed saturation mutagenesis, the mutant shows reduced activity compared to wild-type 749322 4.1.1.25 S101A site-directed saturation mutagenesis, the mutant shows reduced activity compared to wild-type 749322 4.1.1.25 S297A site-directed saturation mutagenesis, the mutant shows reduced activity compared to wild-type 749322 4.1.1.25 S586A site-directed saturation mutagenesis, the mutant variant displays 278% of the catalytic efficiency of the wild-type and increased substrate affinity, which is attributed to decreased steric hindrance and increased hydrophobicity 749322 4.1.1.25 S586C site-directed saturation mutagenesis, inactive mutant 749322 4.1.1.25 S586D site-directed saturation mutagenesis, inactive mutant 749322 4.1.1.25 S586E site-directed saturation mutagenesis, almost inactive mutant 749322 4.1.1.25 S586F site-directed saturation mutagenesis, almost inactive mutant 749322 4.1.1.25 S586G site-directed saturation mutagenesis, the mutant shows reduced activity compared to wild-type 749322 4.1.1.25 S586H site-directed saturation mutagenesis, inactive mutant 749322 4.1.1.25 S586I site-directed saturation mutagenesis, almost inactive mutant 749322 4.1.1.25 S586K site-directed saturation mutagenesis, inactive mutant 749322 4.1.1.25 S586L site-directed saturation mutagenesis, almost inactive mutant 749322 4.1.1.25 S586M site-directed saturation mutagenesis, inactive mutant 749322 4.1.1.25 S586N site-directed saturation mutagenesis, inactive mutant 749322 4.1.1.25 S586P site-directed saturation mutagenesis, inactive mutant 749322 4.1.1.25 S586Q site-directed saturation mutagenesis, inactive mutant 749322 4.1.1.25 S586R site-directed saturation mutagenesis, inactive mutant 749322 4.1.1.25 S586T site-directed saturation mutagenesis, the mutant highly shows reduced activity compared to wild-type 749322 4.1.1.25 S586V site-directed saturation mutagenesis, the mutant highly shows reduced activity compared to wild-type 749322 4.1.1.25 S586W site-directed saturation mutagenesis, inactive mutant 749322 4.1.1.25 S586Y site-directed saturation mutagenesis, inactive mutant 749322 4.1.1.25 T103A site-directed saturation mutagenesis, the mutant shows reduced activity compared to wild-type 749322 4.1.1.25 T298A site-directed saturation mutagenesis, the mutant shows reduced activity compared to wild-type 749322 4.1.1.25 V294A site-directed saturation mutagenesis, the mutant shows reduced activity compared to wild-type 749322 4.1.1.25 V396A site-directed saturation mutagenesis, the mutant shows reduced activity compared to wild-type 749322 4.1.1.25 Y331A site-directed saturation mutagenesis, the mutant shows reduced activity compared to wild-type 749322 4.1.1.25 Y395A site-directed saturation mutagenesis, the mutant shows reduced activity compared to wild-type 749322 4.1.1.25 Y398A site-directed saturation mutagenesis, almost inactive mutant 749322 4.1.1.25 Y398F site-directed saturation mutagenesis, the mutant shows highly reduced activity compared to wild-type 749322 4.1.1.25 Y420A site-directed mutagenesis, inactive mutant 749322 4.1.1.25 Y420F site-directed mutagenesis, inactive mutant 749322