3.5.4.13 E138A no enzymic activity, no change in overall structure compared to wild-type 669375 3.5.4.13 E138A site-directed mutagenesis, inactive mutant, dTTP is bound to the active site of E138A, the region between Val120 and His125 is formed in a new conformation, the C-terminal fold is disordered 686667 3.5.4.13 E138D site-directed mutagenesis, the mutant enzyme shows a 140fold reduction in kcat and altered dTTP inhibition compared to the wild-type enzyme 684700 3.5.4.13 E138Q no enzymic activity, no change in overall structure compared to wild-type 669375 3.5.4.13 H121A site-directed mutagenesis, inactive mutant, dTTP is bound to the active site of E138A, the region between Val120 and His125 is formed in a new conformation, the C-terminal fold is disordered 686667 3.5.4.13 R115A no enzymic activity, no change in overall structure compared to wild-type 669375 3.5.4.13 R115Q site-directed mutagenesis, inactive mutant 684700 3.5.4.13 S111C site-directed mutagenesis, inactive mutant 684700 3.5.4.13 S111T site-directed mutagenesis, the mutant enzyme shows a 30fold reduction in kcat and altered dTTP inhibition compared to the wild-type enzyme, modeling of the active site of the S111T enzyme, overview 684700 3.5.4.13 V122G site-directed mutagenesis, inactive mutant 686667