3.1.3.78 C438S site-directed mutagenesis 697606 3.1.3.78 C462A recombinant protein 697201 3.1.3.78 C462S does not restore the ability of Salmonella to eliminate phosphatidylinositol 4,5-bisphosphate in SigD-deficient bacteria 700351 3.1.3.78 C462S no functional or morphological changes in epithelial cells transfected with phosphatase-deficient mutant C462S 681168 3.1.3.78 DELTA1-135-DELTA118-142 recombinant protein, Saccharomyces cerevisiae: no effect in Saccharomyces cerevisiae, HeLa: no effect (Green Fluorescent Protein linked) in HeLa 697201 3.1.3.78 DELTA1-165 recombinant protein, Saccharomyces cerevisiae: strong impact on actin filaments and budding, HeLa (Green Fluorescent Protein linked): loss of adherence and strong impact on actin filaments 697201 3.1.3.78 DELTA1-351 recombinant protein, Saccharomyces cerevisiae: strong impact on actin filaments and budding, HeLa (Green Fluorescent Protein linked): loss of adherence and strong impact on actin filaments 697201 3.1.3.78 DELTA118-142 recombinant protein, Saccharomyces cerevisiae: strong impact on actin filaments and budding, residues 118–142 crucial for toxicity of SigD in the yeast cells, affecting both phosphatase activity and actin depolarization events, no effect (Green Fluorescent Protein linked) in HeLa 697201 3.1.3.78 DELTA352-563 recombinant protein, no effect in Saccharomyces cerevisiae, no effect in HeLa (Green Fluorescent Protein linked) 697201 3.1.3.78 K527A restores the ability of Salmonella to eliminate phosphatidylinositol 4,5-bisphosphate in SigD-deficient bacteria 700351 3.1.3.78 K530A restores the ability of Salmonella to eliminate phosphatidylinositol 4,5-bisphosphate in SigD-deficient bacteria 700351 3.1.3.78 additional information expression of isozyme I siRNA in HeLa cells does not affect phosphatidylinositol-4,5-bisphosphate levels or EGFR degradation 666740 3.1.3.78 additional information expression of isozyme II siRNA in HeLa cells does not affect phosphatidylinositol-4,5-bisphosphate levels or EGFR degradation 666740 3.1.3.78 R468A recombinant protein, Saccharomyces cerevisiae: strong impact on actin filaments and budding, HeLa (Green Fluorescent Protein linked): loss of adherence and strong impact on actin filaments 697201