3.1.3.75	C87A	involved in a non-essential intramolecular disulfide bond	706604	
3.1.3.75	C94A	involved in a non-essential intramolecular disulfide bond	706604	
3.1.3.75	D123N	site-directed mutagenesis, the mutant enzyme fragment shows reduced activity compared to the wild-type enzyme fragment	664211	
3.1.3.75	D203S	site-directed mutagenesis, inactive mutant enzyme fragment	664211	
3.1.3.75	D262E	low PChP activity	679380	
3.1.3.75	D262E	mutant shows activity less than 2% with respect to native recombinant enzyme	696420	
3.1.3.75	D265E	enzyme activity reduced to 30-35% of wild-type activity	679380	
3.1.3.75	D267E	low PChP activity	679380	
3.1.3.75	D267E	mutant shows activity less than 2% with respect to native recombinant enzyme and is not activated by Cu2+	696420	
3.1.3.75	D31E	protein expression, but no enzyme activity	679380	
3.1.3.75	D32N	site-directed mutagenesis, inactive mutant enzyme fragment	664211	
3.1.3.75	D33E	protein expression, but no enzyme activity	679380	
3.1.3.75	D43N	site-directed mutagenesis, the mutant enzyme fragment shows reduced activity compared to the wild-type enzyme fragment	664211	
3.1.3.75	D43N/D123N	site-directed mutagenesis, inactive mutant enzyme fragment	664211	
3.1.3.75	E42A	site-directed mutagenesis	714357	
3.1.3.75	E43A	site-directed mutagenesis	714357	
3.1.3.75	G261A	reduced activity as compared to wild-type	679380	
3.1.3.75	K242Q	is inactive	679380	
3.1.3.75	K242R	functional enzyme	679380	
3.1.3.75	additional information	generation of phospho1-R74X-null mutant, i.e. Phospho1-/-, mice by N-ethyl-N-nitrosourea mutagenesis	-, 714633, 715704	
3.1.3.75	additional information	site-directed mutagenesis of the aspartyl, D31, D33, and threonyl, T35, residues of motif I, of the seryl, S166, residue of motif II, and of the lysyl, K242, glycyl, G261, and aspartyl residues, D262, D265, and D267, of motif III	714872	
3.1.3.75	additional information	Tn5-751 transposon mutagenesis is used to construct an enzyme-deficient, inactive mutant JUF8-00 of Pseudomonas aeruginosa, the mutant can be complemented by expression of the wild-type enzyme	664697	
3.1.3.75	S166T	mutant shows activity less than 2% with respect to native recombinant enzyme	696420	
3.1.3.75	S166T	reduced PChP activity	679380	
3.1.3.75	T35S	mutant shows activity less than 2% with respect to native recombinant enzyme	696420	
3.1.3.75	T35S	protein expression, low PChP activity	679380	
3.1.3.75	Y82A/Y83A/Y84A	site-directed mutagenesis	714357